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Fluorescence Filters

  • Introduction

Fluorescence Filters


Fluorescent filters are a class of filters classified by application type.


Fluorescence filter is a fluorescence imaging filter for biomedical and life science instruments, the key components, the main role is in the biomedical fluorescence analysis system for the separation and selection of substances in the excitation and emission fluorescence Of the spectral characteristics of the band. It is usually required that the filter cut-off depth be greater than OD5 (optical density, OD = -lgT). The core requirements for filters used in fluorescence detection systems are high cut-off steepness, high transmittance, high positioning accuracy, high cut-off depth, and excellent environmental stability.


Fluorescent filters


Fluorescent filter is a combination of three, three are excitation filters, emission filters and dichroic filters.


Excitation Filter (Exciter Filter, Excitation Filter, Excitation Filter): In the fluorescence microscope, only the excitation wavelength of the filter can pass through the fluorescence. In the past, a short-pass filter was used, and now a band-pass filter is basically used. The housing is marked with arrows indicating the direction of propagation of the recommended light.


Emission Filter (Emitter Filter, Emitter): Select and transmit the fluorescence emitted by the sample, the other range of light cut-off. The wavelength of the emitted light is longer than the wavelength of the excitation light (closer to red). A band-pass filter or a long-wave-pass filter may be selected as the emission filter. The housing is marked with arrows indicating the direction of propagation of the recommended light.


Dichroic Mirror (Dichromic Beamsplitter, Dichromatic Beamsplitter): also known as dichroic mirrors or dichroic mirrors. And placed at an angle of 45 ° to the optical path of the microscope. This filter reflects one color of light (excitation light) and transmits another color of light (emitted light), the reflectivity of the excitation light is greater than 90% and the transmittance of the emitted light is greater than 90%. The impervious portion of the spectrum is reflected rather than absorbed. Filter in the transmitted light and reflected light color complement each other, and thus also known as dichroic filters.


Our fluorescence filters are designed for fluorescence imaging applications, with durability in mind and high-performance optical specifications in manufacturing. The filter substrate is made of quartz, which can achieve 1/10 lambda surface accuracy, while the thermal expansion coefficient of quartz is relatively small, can obtain higher image quality.

Fluorescence Bandpass Filter


Fluorescence Bandpass Filter

CWL(nm) FWHM(nm) Transmission (%) Optical Density Blocking Wavelength (nm) Transmitted Wavefront  Error Material Diameter
340 29 80 ≥6.0 362 - 580 1/4λ UV Fused Silica 12.5 25
357 48 80 ≥6.0 250 - 320 1/4λ UV Fused Silica 12.5 25
370 40 80 ≥6.0 250 - 340 & 394 - 800 1/4λ UV Fused Silica 12.5 25
377 54 85 ≥6.0 409 - 701 1/4λ UV Fused Silica 12.5 25
386 27 90 ≥6.0 300 - 360 & 510 - 700 1/4λ UV Fused Silica 12.5 25
387 15 90 ≥6.0 405 - 700 1/4λ UV Fused Silica 12.5 25
390 45 90 ≥6.0 426.5 - 474.5 1/4λ UV Fused Silica 12.5 25
435 48 93 ≥6.0 505 - 850 1/4λ UV Fused Silica 12.5 25
438 28 93 ≥6.0 250 - 415 & 460 - 600 1/4λ UV Fused Silica 12.5 25
440 46 93 ≥6.0 381.5 - 392.5 1/4λ UV Fused Silica 12.5 25
447 65 93 ≥6.0 490 - 800 1/4λ UV Fused Silica 12.5 25
448 25 93 ≥6.0 250 - 423 & 471 - 950 1/4λ UV Fused Silica 12.5 25
452 53 93 ≥6.0 370 - 410 1/4λ UV Fused Silica 12.5 25
466 45 93 ≥6.0 500 - 550 1/4λ UV Fused Silica 12.5 25
472 35 93 ≥6.0 250 - 442 & 498 - 640 1/4λ UV Fused Silica 12.5 25
475 53 93 ≥6.0 515.5 - 565.5 1/4λ UV Fused Silica 12.5 25
486 36 93 ≥6.0 250 - 455 & 510 - 660 1/4λ UV Fused Silica 12.5 25
494 25 93 ≥6.0 300 - 473 & 518 - 680 1/4λ UV Fused Silica 12.5 25
500 29 93 ≥6.0 250 - 473 & 525 - 670 1/4λ UV Fused Silica 12.5 25
510 25 93 ≥6.0 300 - 490 & 531 - 800 1/4λ UV Fused Silica 12.5 25
512 30 93 ≥6.0 300 - 480 & 540 - 950 1/4λ UV Fused Silica 12.5 25
520 41 93 ≥6.0 250 - 488 & 560 - 700 1/4λ UV Fused Silica 12.5 25
525 18 93 ≥6.0 403 - 455 1/4λ UV Fused Silica 12.5 25
527 22 93 ≥6.0 300 - 513 & 546 - 740 1/4λ UV Fused Silica 12.5 25
530 62 93 ≥6.0 400 - 485 & 569 - 750 1/4λ UV Fused Silica 12.5 25
534 25 93 ≥6.0 300 - 510 & 555 - 725 1/4λ UV Fused Silica 12.5 25
535 48 93 ≥6.0 250 - 497 & 588 - 1000 1/4λ UV Fused Silica 12.5 25
540 56 93 ≥6.0 450 - 500 1/4λ UV Fused Silica 12.5 25
543 27 93 ≥6.0 250 - 518 & 565 - 750 1/4λ UV Fused Silica 12.5 25
549 21 93 ≥6.0 300 - 530 & 568 - 850 1/4λ UV Fused Silica 12.5 25
550 100 93 ≥6.0 300 - 488 & 622 - 975 1/4λ UV Fused Silica 12.5 25
560 32 93 ≥6.0 589.5 - 623.5 1/4λ UV Fused Silica 12.5 25
561 21 93 ≥6.0 582 - 636 1/4λ UV Fused Silica 12.5 25
572 33 93 ≥6.0 250 - 547 & 602 - 925 1/4λ UV Fused Silica 12.5 25
575 35 93 ≥6.0 300 - 541 & 638 - 800 1/4λ UV Fused Silica 12.5 25
578 22 93 ≥6.0 300 - 558 & 598 - 850 1/4λ UV Fused Silica 12.5 25
585 40 93 ≥6.0 300 - 556 & 618 - 780 1/4λ UV Fused Silica 12.5 25
592 49 93 ≥6.0 440 - 555 & 631 - 725 1/4λ UV Fused Silica 12.5 25
605 22 93 ≥6.0 403 - 455 1/4λ UV Fused Silica 12.5 25
615 26 93 ≥6.0 300 - 592 & 638 - 850 1/4λ UV Fused Silica 12.5 25
620 60 93 ≥6.0 300 - 581 & 664 - 850 1/4λ UV Fused Silica 12.5 25
628 38 93 ≥6.0 250 - 598 & 659 - 925 1/4λ UV Fused Silica 12.5 25
640 20 93 ≥6.0 250 - 610 & 655 - 925 1/4λ UV Fused Silica 12.5 25
648 20 93 ≥6.0 300 - 621 & 677 - 925 1/4λ UV Fused Silica 12.5 25
655 47 93 ≥6.0 403 - 455 1/4λ UV Fused Silica 12.5 25
661 26 93 ≥6.0 300 - 638 & 684 - 850 1/4λ UV Fused Silica 12.5 25
676 36 93 ≥6.0 250 - 650 1/4λ UV Fused Silica 12.5 25
692 47 93 ≥6.0 520 - 662 & 733 - 900 1/4λ UV Fused Silica 12.5 25
710 47 93 ≥6.0 250 - 666 & 745 - 940 1/4λ UV Fused Silica 12.5 25
716 47 93 ≥6.0 535 - 682 & 758 - 900 1/4λ UV Fused Silica 12.5 25
769 48 93 ≥6.0 250 - 724 & 808 - 1025 1/4λ UV Fused Silica 12.5 25
775 54 93 ≥6.0 250 - 350, 550 - 737, & 837 - 975 1/4λ UV Fused Silica 12.5 25
785 71 93 ≥6.0 450 - 738 & 830 - 1050 1/4λ UV Fused Silica 12.5 25
832 45 93 ≥6.0 550 - 794 & 878 - 1075 1/4λ UV Fused Silica 12.5 25

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